anti serca2 atpase (Novus Biologicals)
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anti serca2 atpase
Anti Serca2 Atpase, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iid8/SERCA2+ATPase+Antibody+(IID8)+%5BAllophycocyanin%5D/pm40069352-73-136-145
Average 93 stars, based on 1 article reviews
Anti Serca2 Atpase, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iid8/SERCA2+ATPase+Antibody+(IID8)+%5BAllophycocyanin%5D/pm40069352-73-136-145
Average 93 stars, based on 1 article reviews
anti serca2 atpase - by Bioz Stars,
2026-10
93/100 stars
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Immunocytochemistry:Article Title: Induction of MASH-like pathogenesis in the Nwd1 −/− mouse liver Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-Flag-SERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3 × Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr) was used for ER imaging. .. The following primary antibodies were used: anti-Nwd1 (rabbit polyclonal ; 1:400 for immunocytochemistry [ICC]), anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, anti-F4/80 (rabbit polyclonal, #28463-1-AP, RRID: AB_2881149, Proteintech, Rosemont, IL, USA; 1:800 for IHC), Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for ICC, 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-SERCA2 ATPase Clone Article Title: Induction of MASH-like pathogenesis in the Nwd1 -/- mouse liver. Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-FlagSERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3 × Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr)27 was used for ER imaging. .. The following primary antibodies were used: anti-Nwd1 (rabbit polyclonal19; 1:400 for immunocytochemistry [ICC]), anti-cleaved caspase3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, anti-F4/80 (rabbit polyclonal, #28463-1-AP, RRID: AB_2881149, Proteintech, Rosemont, IL, USA; 1:800 for IHC), Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN Communications Biology | (2025) 8:348 3 (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for ICC, 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-SERCA2 ATPase Clone Article Title: Induction of NASH and vacuolar structures in the Nwd1 −/− mouse liver via SERCA2-dependent endoplasmic reticulum stress Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-Flag-SERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3×Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr) [ ] was used for ER imaging. .. The following primary antibodies were used: anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for immunocytochemistry [ICC], 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:250 for IHC, 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-Nwd1 (rabbit polyclonal generated via immunization with the recombinant mouse Nwd1 protein [ ]; 1:400 for ICC), anti-SERCA2 ATPase Clone Immunohistochemistry:Article Title: Induction of MASH-like pathogenesis in the Nwd1 −/− mouse liver Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-Flag-SERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3 × Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr) was used for ER imaging. .. The following primary antibodies were used: anti-Nwd1 (rabbit polyclonal ; 1:400 for immunocytochemistry [ICC]), anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, anti-F4/80 (rabbit polyclonal, #28463-1-AP, RRID: AB_2881149, Proteintech, Rosemont, IL, USA; 1:800 for IHC), Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for ICC, 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-SERCA2 ATPase Clone Article Title: Induction of MASH-like pathogenesis in the Nwd1 -/- mouse liver. Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-FlagSERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3 × Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr)27 was used for ER imaging. .. The following primary antibodies were used: anti-Nwd1 (rabbit polyclonal19; 1:400 for immunocytochemistry [ICC]), anti-cleaved caspase3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, anti-F4/80 (rabbit polyclonal, #28463-1-AP, RRID: AB_2881149, Proteintech, Rosemont, IL, USA; 1:800 for IHC), Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN Communications Biology | (2025) 8:348 3 (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for ICC, 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-SERCA2 ATPase Clone Article Title: Induction of NASH and vacuolar structures in the Nwd1 −/− mouse liver via SERCA2-dependent endoplasmic reticulum stress Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-Flag-SERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3×Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr) [ ] was used for ER imaging. .. The following primary antibodies were used: anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for immunocytochemistry [ICC], 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:250 for IHC, 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-Nwd1 (rabbit polyclonal generated via immunization with the recombinant mouse Nwd1 protein [ ]; 1:400 for ICC), anti-SERCA2 ATPase Clone Western Blot:Article Title: Induction of MASH-like pathogenesis in the Nwd1 −/− mouse liver Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-Flag-SERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3 × Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr) was used for ER imaging. .. The following primary antibodies were used: anti-Nwd1 (rabbit polyclonal ; 1:400 for immunocytochemistry [ICC]), anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, anti-F4/80 (rabbit polyclonal, #28463-1-AP, RRID: AB_2881149, Proteintech, Rosemont, IL, USA; 1:800 for IHC), Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for ICC, 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-SERCA2 ATPase Clone Article Title: Induction of MASH-like pathogenesis in the Nwd1 -/- mouse liver. Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-FlagSERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3 × Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr)27 was used for ER imaging. .. The following primary antibodies were used: anti-Nwd1 (rabbit polyclonal19; 1:400 for immunocytochemistry [ICC]), anti-cleaved caspase3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, anti-F4/80 (rabbit polyclonal, #28463-1-AP, RRID: AB_2881149, Proteintech, Rosemont, IL, USA; 1:800 for IHC), Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN Communications Biology | (2025) 8:348 3 (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for ICC, 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-SERCA2 ATPase Clone Article Title: Induction of NASH and vacuolar structures in the Nwd1 −/− mouse liver via SERCA2-dependent endoplasmic reticulum stress Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-Flag-SERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3×Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr) [ ] was used for ER imaging. .. The following primary antibodies were used: anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for immunocytochemistry [ICC], 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:250 for IHC, 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-Nwd1 (rabbit polyclonal generated via immunization with the recombinant mouse Nwd1 protein [ ]; 1:400 for ICC), anti-SERCA2 ATPase Clone Generated:Article Title: Induction of NASH and vacuolar structures in the Nwd1 −/− mouse liver via SERCA2-dependent endoplasmic reticulum stress Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-Flag-SERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3×Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr) [ ] was used for ER imaging. .. The following primary antibodies were used: anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for immunocytochemistry [ICC], 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:250 for IHC, 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-Nwd1 (rabbit polyclonal generated via immunization with the recombinant mouse Nwd1 protein [ ]; 1:400 for ICC), anti-SERCA2 ATPase Clone Recombinant:Article Title: Induction of NASH and vacuolar structures in the Nwd1 −/− mouse liver via SERCA2-dependent endoplasmic reticulum stress Article Snippet: The deletion mutants 3×Flag-SERCA2-N (amino acids 1–787, hereafter called Flag-Flag-SERCA2-N) and 3×Flag-SERCA2-C (amino acids 788–1044, hereafter called Flag-SERCA2-C) were constructed from 3×Flag-SERCA2 using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). mCherry-Sec61B (Addgene, plasmid # 121160, deposited by Dr. Mayr) [ ] was used for ER imaging. .. The following primary antibodies were used: anti-cleaved caspase-3 (Asp175) (rabbit polyclonal, #9661, RRID: AB_2341188, Cell Signaling Technology, Danvers, MA, USA; 1:400 for immunohistochemistry [IHC]), anti-cleaved caspase-1 (Ala317) p10 (rabbit polyclonal, #AF4022, RRID: AB_2845464, Affinity Biosciences, Cincinnati, OH, USA; 1:200 for IHC), anti-BSCL2/SEIPIN (rabbit polyclonal, #ab106793, RRID: AB_10974250, Abcam, Cambridge, UK; 1:200 for IHC, 1:1000 for western blotting [WB]), anti-KDEL (mouse monoclonal, #M181-3, RRID: AB_10693914, MBL, Woburn, MA, USA; 1:400 for IHC, 1:400 for immunocytochemistry [ICC], 1:5000 for WB), anti-LAMP1 (1D4B) (rat monoclonal, #sc-19992, RRID: AB_2134495, Santa Cruz Biotechnology, Dallas, TX, USA; 1:250 for IHC, 1:1000 for WB), anti-LC3A/B (D3U4C) XP (rabbit monoclonal, #12741, RRID: AB_2617131, Cell Signaling Technology; 1:200 for IHC, 1:2000 for WB), anti-Nwd1 (rabbit polyclonal generated via immunization with the recombinant mouse Nwd1 protein [ ]; 1:400 for ICC), anti-SERCA2 ATPase Clone |